Comparative Study of Captopril Derivatization Reaction by LC-UV, LC-MS and CE-UV Methods
نویسندگان
چکیده
The LC-UV, LC-MS and CE-UV study of chemical reaction between captopril and p-bromophenacyl bromide as derivatizing reagent is reported. During transformation of captopril, its thiol group is involved and the reaction is irreversible. Neutral or alkaline environments favor derivatization. The yield of reaction increases linear with the concentration of the reagent, while changes in temperature do not influence it significantly. Kinetic studies show that derivatization of captopril flows quickly, reaching a plateau after 30 minutes. Based on the study of these effects some important parameters such as derivatizing agent concentration, reaction time, temperature and pH were confined. The results were used for development of captopril determination methods both in pharmaceutical formulations and biological samples. (doi: 10.5562/cca1787)
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